The research progress of sericin utility

 

 

    When lee is responsible for the roots (310029) in hangzhou, zhejiang province, zhejiang university college of animal science,

 

 

 

    Pick to discusses the sericin in the production of high-performance biological material, antioxidation, improving culture cell, freeze protection research progress, and discusses its application prospects.

 

    Keywords silkworm sericin efficacy

 

    Chinese library classification number: $886.9 document id code: A article number: ISSN0258-4069-01-2004 】 【 0010-04

 

 

    Silkworm mature spit silk gland of silk material and form a cocoon, in order to protect its normalization of pupa and feather. Cocoon layer formed by overlapping adhesive cocoon filament, a silk composed of two parallel monofilament fiber bonding, each monofilament and fibroin and sericin outer form by the center. Sericin on two synthetic a cocoon silk and silk monofilament overlapping into cocoon layer adhesion effect, the liquid fibroin fibrosis and plays a role of lubrication and protection from the spinneret hole traction, out at the time of the silk reeling cocoon silk into silk have cooperation. In addition, for the effect of sericin is poorly understood. Sericin for polymer water soluble proteins, the molecular weight of 10 ~ 300 kda, consists of 18 kinds of amino acids, which have strong polarity such as hydroxyl, carboxyl, amino side base of amino acids accounted for the vast majority. Sericin accounted for about 3 o % of the cocoon silk, silk reeling and refined greatly part was removed. The effective use of such a large number of high quality protein, has been keen to the silk industry. People carried out research for a long time, in the production of high-performance biological material, antioxidant effect, cell culture promotes, freeze protection has made positive progress, this article makes a brief argument, and the deepening of research in this field.

 

L made high performance of biological materials

 

    The sericin mixed with other materials such as resin, can be made into a high-performance creatures such as polyurethane polymer materials. Biological polymeric material is biodegradable, non-polluting, high moisture absorption of moisture exhaust rate, good elastic properties, and because the contains a lot of abandoned sericin, the production cost greatly reduced, showed a strong market competitiveness \"1 l. Sericin crossing with other ingredients, mixing, etc, can be made into polymer film separation. Mizoguchi sericin, hydrochloric acid, etc. (1991) will be cross-linked agent formaldehyde, a mixture of ethyl carbamate, flat out on the glass plate at room temperature, using 120 ~ (2 hot air treatment for 10 min, get about 1301 xm thick film, this film can be effectively isolated from water, alcohol, the mixture of alcohol, and reusable t21. Yamada, etc. (1994) with sericin liquid propylene eyes, calcium sulfate, sodium sulfate of dodecane ester, distilled water mixes liquid mixture, buy 6 o ℃ under fully aggregate 4 h, polymer after filtering, washing, drying, soluble in dimethyl sulfoxide solution OoC), and tile Yu Guangban under 8 o ℃ treatment 2 h, the preparation of polymer film thickness for 301 - m protein, this protein polymer film can be used

 

    Water and effective separation of organic phase [31. (2001), such as Yoshikawa will sericin blended with AGAR and agarose, preparation of the affordable 0. O1-2 KGF/cm pressure porous water permeability of membrane, the membrane can effectively separate ether, ethanol, methanol, a mixture of methyl tert-butyl ether asked. Made of sericin films, placed in a liquid crystal layer can be a uniform distribution of liquid crystal molecules, which can produce the free flow of high quality liquid crystal display (LCD). In addition, the film can also be used for immobilized enzyme production. Miyairi etc. (1978) using glutaraldehyde as crosslinking agent, will B a glycosidase enzymes were fixed on the wire film, produced by the immobilized B a glycosidase enzymes of heat resistance, resistance to electroosmosis force and stability are improved, but the enzyme activity is low. If the village cheng (1992) with sericin solution such as embedding glucose oxidase (GOD), by embedding enzyme showed higher activity, and save the enzyme activity is still up to 8 months after 8 o % above [61. Some synthetic fiber after adding modification of sericin its features can be improved to a great extent. Wakabayashi etc. (1994) reported by sericin modified polyurethane fiber, can make the hygroscopicity of polyamine fiber increased by more than five times, and after more than 5 o time washing, remained more than 85%. Its moisture absorption will be synthetic or natural fiber fabric in 3% silk (cotton)

 

Glue solution impregnation after a certain period of time, in 100 ~ C under dry 3 min, such fabrics containing sericin protective skin damage and inhibit the role of the skin rashes and other skin diseases occur, can be used in diapers, diaper pad production yan, bandages and other products. In addition, the sericin mixed in the rubber, can make the rubber compatibility increased significantly, the skin is allergic to reduce, thus more suitable for as a man-made leather handles for used to produce rubber gloves, sports equipment and other products.

 

2 antioxidant effect

    Sericin can in vitro inhibition lipid peroxidation and activity of tyrosinase, exhibited obvious antioxidant effect, can be used as high-quality natural antioxidants in food, cosmetic and application [91. Sasaki (2000) in the study of drug - induced mice produced by Escherichia in oncology, found that feeding probability of sericin mice colorectal tumor was significantly lower. To investigate the mechanism of sericin can inhibit colorectal oncology generated is difficult to be found, the sericin digestive enzyme decomposition, still can maintain its high activity in the photographed cannibal animal gut, so that the animal intestinal contents also have antioxidant effect, make the colorectal mucosa lipid peroxidation quality is greatly reduced, thereby inhibiting Escherichia produce swollen c9. Through the test and analysis found that the sericin, natural moisturizing factor, amino acid composition and found in human skin is very similar to the (NMF) composed of amino acids. Therefore, sericin can be used for the production of wound dressing. Tsubouchi (1999) by sericin and fibroin mixture made of a non crystalline protein film thickness is 10-130~m, the membrane crystallization degree is lower than 1O%, the density of 1100-1400 kg / m at room temperature is higher than 10%, water solubility, water absorption of up to more than 100 times its own volume n". The film deposited in the wound, the results can accelerate wound healing, and does not need to go to the dressing in the wound healing time, thus avoiding due to dressing and hurt the new skin, showing good practicality. Sericin has anti drug allergy and the role of ultraviolet radiation, sericin can be coated on the skin of mice, significantly inhibited by skin erythema, fold drug induced skin oncology and ultraviolet irradiation induced wrinkles etc.. Find out the mechanism of sericin on the skin, will enable the sericin get more extensive application, deep in the skin care medicine.

 

3 cell culture role

    Terada wise (2002) in the sericin solution added in serum-free culture, to study the effectiveness of sericin on animal cell culture results, sericin can greatly enhance the hybridoma cell proliferation, the enhancement effect with the addition of bovine serum albumin in the same four. More noteworthy is, through high pressure steam sterilization after sericin, still showed the same and filter sterilized sericin, promote cell proliferation effect. The results also show that: in the search for simple, practical method of sterilization in cell cultures, sericin has extremely high application value. Sericin promotes cell proliferation effect, on human cervical cancer Hela cells, Balb3T3 mouse line dimension bud cells, human liver cell line HepG2, used as an animal model of rabbit corneal cells instead of RC4, used for virus expression vector of human fetal kidney cell line 293 was extensive cell line were observed. The sericin can be high pressure sterilization, and from non mammalian, so there won't be a source of infection is unknown, it is the proliferation of animal cells, has the characteristics of high value, high safety etc.. Sericin and fibroin film has excellent permeability, and the functional characteristics and the human cornea, skin, blood vessels of similar. Murase (1994) will cocoon shell is completely dissolved in acetic acid solution 98%, tumbler made into films and repeatedly washed, made of artificial cornea, contact lenses, artificial blood vessel, obtain the ideal effect [l31. Ahn (2001) the sericin and polymerization of acrylic acid, also succeeded in creating a new polymerization skin [] 41.

 

4 frozen protective effect

    Tsujimoto (2001) in the study of gene recombinant sericin, discovered the sericin has a protective effect against Escherichia coli when old cryopreserved cells damage. Sericin gene Serl has a serine repeat sequence characteristic, the repeat sequence (sericin peptide) composed of 38 amino acids, expressed in Escherichia coli in the system, found that the sericin peptide expression strain decreased survival rate in the frozen melted was obviously inhibited, the results showed that the sericin has protective effect on freezing treatment. Freezing thawing processing causes the cell lactate dehydrogenase activity decreased dramatically, the general should be reduced to the original activity below lO%. To investigate the protective effect of freezing on lactate dehydrogenase by sericin peptide refined, found that adding the processing area of sericin peptides, the lactate dehydrogenase activity was not reduced, almost loo% keeping the original activity. Sericin peptide rich in hydrophilic amino acid, hydrophilic amino acids and water molecules combined, can protect cells are dehydrated, thereby inhibiting protein by freezing and the occurrence of the denaturation and inactivation, this is [151 the basic mechanism of sericin has frozen the protective effect. The use of antifreeze properties of sericin, sericin can be protective film is covered on the refrigerator, refrigerator car, cold storage ship refrigeration equipment surface, can play a protective role in the effective freezing. The surface of the skin will sericin paste applied to engage in deep freeze workers, can protect human skin by frost. In addition, sericin can also be applied to highway, roof, can rise to prevent frost and easy to remove snow effect.

   5 prospect

    Sericin in the traditional silk processing industry, is discarded as a waste. However, extensive studies have proved, sericin in functional biomaterials, antioxidant, anti freeze, promote cell proliferation and so on, and has very high application value. Therefore, the development of a new function of sericin and utilized, waste to treasure, to the revitalization of the current in the silk industry in the bottom, it will produce great influence. A new research field of sericin unfolds, also involves the cultivation of new silkworm varieties. Study on agricultural biological resources in Japan by greatly improving the sericin gene expression and closed silk fibroin gene expression, successfully bred only secretion of sericin (nearly 100%) of the silkworm varieties, named "star of sericin". Silkworm and lead the varieties of fibroin structure gene abnormality and secretion of sericin content relatively high naked pupa (Nd) compared to the secretion of the sericin content more than 4 times higher, and the secretion of the sericin purity as high as 98%. The silkworm varieties will be as innovative material used for mass production of high-purity sericin, this will to some extent promote the development of new functions of sericin.

 

 

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